Year 2019, Volume 23, Issue 2, Pages 184 - 192 2019-04-01

Testing The Biodegradability of Priority And Emerging Contaminants As A Mixture

Ulas Tezel [1] , Begüm Şepitci [2]

71 93

Currently about 110,000 chemical substances are present in the European market. The fate of most of those chemicals in the environment is not known. However, biodegradability of those chemicals should be tested before they are registered to the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) System. Current protocols offered by The Organisation for Economic Co-operation and Development (OECD) for testing the biodegradability of the chemicals are limited mainly due to they are low throughput and do not reflect real-world conditions. In OECD protocols, the biodegradability of a single chemical is tested. However, many chemicals coexist in the environment. In addition, experiments are set at a very high initial chemical concentration that is not expected in the environment. Both limitations are due to the lack of an analytical method which can measure multiple compounds simultaneously at very low concentrations. In this study, we coupled OECD 314 Simulation Tests to Assess the Biodegradability of Chemicals Discharged in Wastewater protocol with a powerful liquid chromatography mass spectrometry with scheduled multiple reaction monitoring and tested the biodegradability of 32 priority substances and chemicals with emerging concern. Only seven chemicals were degraded in the test within 28 days. The biodegradation half-lives of those degradable chemicals ranged between 0.6 to 18 days. Acetaminophen was degraded the fastest whereas biodegradation of sulfamethoxazole took longer than the rest of the biodegradable chemicals tested. The novel methodology described here can be applied to test biodegradability of different chemicals as a mixture and adopted as a standard protocol. 

biodegradability, biodegradation testing, chemicals of emerging concern, priority substances, priority substances
  • L. Jones, J. Ronan, B. McHugh, E. McGovern, and F. Regan, "Emerging priority substances in the aquatic environment: a role for passive sampling in supporting WFD monitoring and compliance," Analytical Methods, vol. 7, pp. 7976-7984, 2015.
  • K. Fent, A. A. Weston, and D. Caminada, "Ecotoxicology of human pharmaceuticals," Aquatic Toxicology, vol. 76, pp. 122-159, 2006.
  • A. Pruden, R. T. Pei, H. Storteboom, and K. H. Carlson, "Antibiotic resistance genes as emerging contaminants: Studies in northern Colorado," Environmental Science & Technology, vol. 40, pp. 7445-7450, 2006.
  • K. Czechowska, V. Sentchilo, S. Beggah, S. Rey, M. Seyfried, and J. R. van der Meer, "Examining Chemical Compound Biodegradation at Low Concentrations through Bacterial Cell Proliferation," Environmental Science & Technology, vol. 47, pp. 1913-1921, 2013.
  • OECD, Test No. 301: Ready Biodegradability: OECD Publishing, 1992.
  • OECD, Test No. 302A: Inherent Biodegradability: Modified SCAS Test: OECD Publishing, 1981.
  • OECD, Test No. 314: Simulation Tests to Assess the Biodegradability of Chemicals Discharged in Wastewater: OECD Publishing, 2008.
  • A. Kowalczyk, T. J. Martin, O. R. Price, J. R. Snape, R. A. van Egmond, C. J. Finnegan, et al., "Refinement of biodegradation tests methodologies and the proposed utility of new microbial ecology techniques," Ecotoxicology and Environmental Safety, vol. 111, pp. 9-22, 2015.
  • T. J. Martin, A. K. Goodhead, K. Acharya, I. M. Head, J. R. Snape, and R. J. Davenport, "High Throughput Biodegradation-Screening Test To Prioritize and Evaluate Chemical Biodegradability," Environmental Science & Technology, 2017.
  • B. De Gusseme, L. Vanhaecke, W. Verstraete, and N. Boon, "Degradation of acetaminophen by Delftia tsuruhatensis and Pseudomonas aeruginosa in a membrane bioreactor," Water Research, vol. 45, pp. 1829-1837, 2011.
  • J. Hu, L. L. Zhang, J. M. Chen, and Y. Liu, "Degradation of paracetamol by Pseudomonas aeruginosa strain HJ1012," Journal of Environmental Science and Health Part a-Toxic/Hazardous Substances & Environmental Engineering, vol. 48, pp. 791-799, 2013.
  • S. J. Wu, L. L. Zhang, and J. M. Chen, "Paracetamol in the environment and its degradation by microorganisms," Applied Microbiology and Biotechnology, vol. 96, pp. 875-884, 2012.
  • K. A. Shin and J. C. Spain, "Pathway and Evolutionary Implications of Diphenylamine Biodegradation by Burkholderia sp Strain JS667," Applied and Environmental Microbiology, vol. 75, pp. 2694-2704, 2009.
  • C. Christodoulatos, A. D. Koutsospyros, B. W. Brodman, and G. P. Korfiatis, "Biodegradation of diphenylamine by selected microbial cultures," Journal of Environmental Science and Health Part A-Environmental Science and Engineering & Toxic and Hazardous Substance Control, vol. 32, pp. 15-30, 1997.
  • Y. Yamazaki, Y. Hayashi, M. Arita, T. Hieda, and Y. Mikami, "Microbial Conversion of alpha-Ionone, alpha-Methylionone, and alpha-Isomethylionone," Applied Environmental Microbiology, vol. 54, pp. 2354-60, 1988.
  • R. N. Fathulla, "Aerobic Aquatic Metabolism of 14C-Pyriproxyfen," Sumitomo Chemical Company1993.
  • S. Larcher and V. Yargeau, "Biodegradation of sulfamethoxazole: current knowledge and perspectives," Applied Microbiology and Biotechnology, vol. 96, pp. 309-318, 2012.
  • P. Y. Nguyen, G. Carvalho, A. C. Reis, O. C. Nunes, M. A. M. Reis, and A. Oehmen, "Impact of biogenic substrates on sulfamethoxazole biodegradation kinetics by Achromobacter denitrificans strain PR1," Biodegradation, vol. 28, pp. 205-217, 2017.
  • J. Birkigt, T. Gilevska, B. Ricken, H. H. Richnow, D. Vione, P. F. X. Corvini, et al., "Carbon Stable Isotope Fractionation of Sulfamethoxazole during Biodegradation by Microbacterium sp Strain BR1 and upon Direct Photolysis," Environmental Science & Technology, vol. 49, pp. 6029-6036, 2015.
  • B. C. Jiang, A. Li, D. Cui, R. Cai, F. Ma, and Y. N. Wang, "Biodegradation and metabolic pathway of sulfamethoxazole by Pseudomonas psychrophila HA-4, a newly isolated cold-adapted sulfamethoxazole-degrading bacterium," Applied Microbiology and Biotechnology, vol. 98, pp. 4671-4681, 2014.
  • A. Barra Caracciolo, P. Grenni, R. Ciccoli, G. Di Landa, and C. Cremisini, "Simazine biodegradation in soil: analysis of bacterial community structure by in situ hybridization," Pest Management Science, vol. 61, pp. 863-869, 2005.
  • M. Blaszak, R. Pelech, and P. Graczyk, "Screening of Microorganisms for Biodegradation of Simazine Pollution (Obsolete Pesticide Azotop 50 WP)," Water Air and Soil Pollution, vol. 220, pp. 373-385, 2011.
  • T. Kodama, L. X. Ding, M. Yoshida, and M. Yajima, "Biodegradation of an s-triazine herbicide, simazine," Journal of Molecular Catalysis B-Enzymatic, vol. 11, pp. 1073-1078, 2001.
  • R. Wan, Y. Y. Yang, W. M. Sun, Z. Wang, and S. G. Xie, "Simazine biodegradation and community structures of ammonia-oxidizing microorganisms in bioaugmented soil: impact of ammonia and nitrate nitrogen sources," Environmental Science and Pollution Research, vol. 21, pp. 3175-3181, 2014.
Primary Language en
Subjects Environmental Engineering
Published Date April 2019
Journal Section Research Articles
Authors

Orcid: 0000-0001-8322-1666
Author: Ulas Tezel (Primary Author)
Institution: BOGAZICI UNIVERSITY, INSTITUTE OF ENVIRONMENTAL SCIENCES
Country: Turkey


Orcid: 0000-0001-8172-7524
Author: Begüm Şepitci
Institution: BOGAZICI UNIVERSITY, INSTITUTE OF ENVIRONMENTAL SCIENCES
Country: Turkey


Bibtex @research article { saufenbilder413597, journal = {Sakarya University Journal of Science}, issn = {1301-4048}, eissn = {2147-835X}, address = {Sakarya University}, year = {2019}, volume = {23}, pages = {184 - 192}, doi = {10.16984/saufenbilder.413597}, title = {Testing The Biodegradability of Priority And Emerging Contaminants As A Mixture}, key = {cite}, author = {Tezel, Ulas and Şepitci, Begüm} }
APA Tezel, U , Şepitci, B . (2019). Testing The Biodegradability of Priority And Emerging Contaminants As A Mixture. Sakarya University Journal of Science, 23 (2), 184-192. DOI: 10.16984/saufenbilder.413597
MLA Tezel, U , Şepitci, B . "Testing The Biodegradability of Priority And Emerging Contaminants As A Mixture". Sakarya University Journal of Science 23 (2019): 184-192 <http://www.saujs.sakarya.edu.tr/issue/39539/413597>
Chicago Tezel, U , Şepitci, B . "Testing The Biodegradability of Priority And Emerging Contaminants As A Mixture". Sakarya University Journal of Science 23 (2019): 184-192
RIS TY - JOUR T1 - Testing The Biodegradability of Priority And Emerging Contaminants As A Mixture AU - Ulas Tezel , Begüm Şepitci Y1 - 2019 PY - 2019 N1 - doi: 10.16984/saufenbilder.413597 DO - 10.16984/saufenbilder.413597 T2 - Sakarya University Journal of Science JF - Journal JO - JOR SP - 184 EP - 192 VL - 23 IS - 2 SN - 1301-4048-2147-835X M3 - doi: 10.16984/saufenbilder.413597 UR - https://doi.org/10.16984/saufenbilder.413597 Y2 - 2018 ER -
EndNote %0 Sakarya University Journal of Science Testing The Biodegradability of Priority And Emerging Contaminants As A Mixture %A Ulas Tezel , Begüm Şepitci %T Testing The Biodegradability of Priority And Emerging Contaminants As A Mixture %D 2019 %J Sakarya University Journal of Science %P 1301-4048-2147-835X %V 23 %N 2 %R doi: 10.16984/saufenbilder.413597 %U 10.16984/saufenbilder.413597
ISNAD Tezel, Ulas , Şepitci, Begüm . "Testing The Biodegradability of Priority And Emerging Contaminants As A Mixture". Sakarya University Journal of Science 23 / 2 (April 2019): 184-192. https://doi.org/10.16984/saufenbilder.413597