Yıl 2018, Cilt 22, Sayı 6, Sayfalar 1804 - 1811 2018-12-01

Türkiye’de Bulunan Bazı Sıcak Su Kaynaklarından İzole Edilen Bakterilerin Ksilanaz ve Glukoz İzomeraz Üretimi Yönünden Taranması
Screening of Xylanase and Glucose Isomerase Producing Bacteria Isolated from Hot Springs in Turkey

Dilsat Nigar Colak [1] , Kadriye INAN BEKTAS [2] , Muslum TOKGOZ [3] , Sabriye CANAKCI [4] , Ali Osman BELDUZ [5]

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Bu çalışmanın amacı Türkiye’nin Ege Bölgesinde bulunan bazı sıcak su kaynaklarından elde edilen termofilik bakterilerin ksilanaz ve glukoz izomeraz üretmi yönünden taranmasıdır. Toplam 68 izolat (Anoxybacillus, Brevibacillus, Geobacillus, Aneurinibacillus, Thermus, Paenibacillus ve Proteobacter) daha önceden bu alanlardan temin edilmiş ve 16S rDNA gen dizilerine göre tanımlanmıştır. Öncelikle petri deneyi ile taranan izolatlar, ilgili enzimlere uygun besiyeri içerisinde ksilanaz için iki gün, glukoz izomeraz için ise 5-6 gün süreyle 50-60 °C’de inkübasyona tabii tutuldu ve pozitif izolatlar belirlendi. Enzim ürettiği belirlenen türler doğrulama için spektrofotometrik analizlere tabi tutuldu, birchwood ksilan ve glukoz substrat olarak kullanıldı. Türlerin çoğunluğu (59 tanesi) ksilanaz aktivitesi yönünden pozitif iken 16 türün de glukoz izomeraz aktivitesine sahip olduğu gözlendi. Izolatlardan 14 tanesinin her iki enzim yönünden de aktif olduğu belirlendi. Paenibacillus, Aneurinibacillus ve Proteobacter türlerinden hiç bir tanesi glukoz izomeraz aktivitesi göstermezken Geobacillus türlerinin glukoz izomeraz aktivitesinin oldukça yüksek olduğu belirlendi. Her iki enzim de aktivitelerini, biyoteknolojik uygulamalar için tercih edilen sıcaklık aralığı olan 50-60 °C’de gösterdi.

The aim of this study was screening of xylanase and glucose isomerase producing thermophilic bacteria isolated from some hot springs located to Aegean Region of Turkey. Total sixty eight thermophilic isolates (Anoxybacillus, Brevibacillus, Geobacillus, Aneurinibacillus, Thermus, Paenibacillus and Proteobacter) were collected previously from these fields and identified based on 16S rDNA gene sequences. The isolates were screened with plate assay for determining the xylanase and glucose isomerase production abilities seperately in order to find new strains for industrial processes. After an incubation period of two days for xylanase and 5-6 days for glucose isomerase at 50-60 °C, positive strains were determined. Enzyme producing strains were confirmed by spectrophotemetric measurements with crude enzyme extracts, birchwood xylan and glucose were used as substrates. Most of the strains (59 strains) were positive for xylan degradation while only sixteen of the strains had showed glucose isomerase activity. Fourteen of the strains have showed both xylanase and glucose isomerase activity. None of the Paenibacillus, Aneurinibacillus, and Proteobacter strains were glucose isomerase positive, although the glucose isomerase activity of Geobacillus strains were notably high. Both xylanase and glucose isomerase activities have observed at 50-60 °C which is suitable for biotechnological applications.

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Birincil Dil en
Konular Biyoloji
Yayımlanma Tarihi December 2018
Dergi Bölümü Araştırma Makalesi
Yazarlar

Yazar: Dilsat Nigar Colak (Sorumlu Yazar)
Kurum: GİRESUN ÜNİVERSİTESİ
Ülke: Turkey


Yazar: Kadriye INAN BEKTAS
Kurum: KARADENİZ TEKNİK ÜNİVERSİTESİ
Ülke: Turkey


Yazar: Muslum TOKGOZ
Ülke: Turkey


Yazar: Sabriye CANAKCI
Kurum: KARADENİZ TEKNİK ÜNİVERSİTESİ
Ülke: Turkey


Yazar: Ali Osman BELDUZ
Kurum: KARADENİZ TEKNİK ÜNİVERSİTESİ
Ülke: Turkey


Bibtex @araştırma makalesi { saufenbilder356720, journal = {Sakarya University Journal of Science}, issn = {1301-4048}, eissn = {2147-835X}, address = {Sakarya Üniversitesi}, year = {2018}, volume = {22}, pages = {1804 - 1811}, doi = {10.16984/saufenbilder.356720}, title = {Screening of Xylanase and Glucose Isomerase Producing Bacteria Isolated from Hot Springs in Turkey}, key = {cite}, author = {CANAKCI, Sabriye and TOKGOZ, Muslum and INAN BEKTAS, Kadriye and Colak, Dilsat Nigar and BELDUZ, Ali Osman} }
APA Colak, D , INAN BEKTAS, K , TOKGOZ, M , CANAKCI, S , BELDUZ, A . (2018). Screening of Xylanase and Glucose Isomerase Producing Bacteria Isolated from Hot Springs in Turkey. Sakarya University Journal of Science, 22 (6), 1804-1811. DOI: 10.16984/saufenbilder.356720
MLA Colak, D , INAN BEKTAS, K , TOKGOZ, M , CANAKCI, S , BELDUZ, A . "Screening of Xylanase and Glucose Isomerase Producing Bacteria Isolated from Hot Springs in Turkey". Sakarya University Journal of Science 22 (2018): 1804-1811 <http://www.saujs.sakarya.edu.tr/issue/31266/356720>
Chicago Colak, D , INAN BEKTAS, K , TOKGOZ, M , CANAKCI, S , BELDUZ, A . "Screening of Xylanase and Glucose Isomerase Producing Bacteria Isolated from Hot Springs in Turkey". Sakarya University Journal of Science 22 (2018): 1804-1811
RIS TY - JOUR T1 - Screening of Xylanase and Glucose Isomerase Producing Bacteria Isolated from Hot Springs in Turkey AU - Dilsat Nigar Colak , Kadriye INAN BEKTAS , Muslum TOKGOZ , Sabriye CANAKCI , Ali Osman BELDUZ Y1 - 2018 PY - 2018 N1 - doi: 10.16984/saufenbilder.356720 DO - 10.16984/saufenbilder.356720 T2 - Sakarya University Journal of Science JF - Journal JO - JOR SP - 1804 EP - 1811 VL - 22 IS - 6 SN - 1301-4048-2147-835X M3 - doi: 10.16984/saufenbilder.356720 UR - http://dx.doi.org/10.16984/saufenbilder.356720 Y2 - 2018 ER -
EndNote %0 Sakarya University Journal of Science Screening of Xylanase and Glucose Isomerase Producing Bacteria Isolated from Hot Springs in Turkey %A Dilsat Nigar Colak , Kadriye INAN BEKTAS , Muslum TOKGOZ , Sabriye CANAKCI , Ali Osman BELDUZ %T Screening of Xylanase and Glucose Isomerase Producing Bacteria Isolated from Hot Springs in Turkey %D 2018 %J Sakarya University Journal of Science %P 1301-4048-2147-835X %V 22 %N 6 %R doi: 10.16984/saufenbilder.356720 %U 10.16984/saufenbilder.356720
ISNAD Colak, Dilsat Nigar , INAN BEKTAS, Kadriye , TOKGOZ, Muslum , CANAKCI, Sabriye , BELDUZ, Ali Osman . "Türkiye’de Bulunan Bazı Sıcak Su Kaynaklarından İzole Edilen Bakterilerin Ksilanaz ve Glukoz İzomeraz Üretimi Yönünden Taranması". Sakarya University Journal of Science 22 / 6 (Aralık 2018): 1804-1811. http://dx.doi.org/10.16984/saufenbilder.356720