Yıl 2018, Cilt 22, Sayı 6, Sayfalar 1585 - 1590 2018-12-01

An Investigation of Inhibition Effects of Honey, Pollen, Propolis and Royal Jelly Extracts on Thioredoxin Reductase Enzyme Activity
Tiyoredoksin Redüktaz Enzim Aktivitesi Üzerine Bal, Polen, Propolis ve Arı Sütü Extraktlarının İnhibisyon Etkilerinin Araştırılması

Ebru AKKEMİK [1] , Gamze AKBULUT [2]

321 325

The thioredoxin reductase enzyme is an enzyme that prevents the mechanism of apoptosis from working and thus triggers the formation of cancer. Therefore, the inhibition of the thioredoxin reductase enzyme is thought to prevent or inhibit cancer. In this study, the effects of extracts of plateau honey, pine honey, chestnut honey, mad or wild honey, pollen, propolis and royal jelly on thioredoxin reductase enzyme activity were investigated. Enzyme activities were measured at constant substrate and different inhibitor concentrations to calculate IC50 values. Over five different inhibitor concentrations were used for measuring the inhibition constant. Total antioxidant activity were investigated in order to compare the extracts used in the inhibition study. The strongest inhibitory effect was seen in the pollen methanol extract (IC50 = 2.44μg /mL)

Tiyoredoksin redüktaz enzimi, apoptoz mekanizmasının çalışmasını önleyen ve böylece kanser oluşumunu tetikleyen bir enzimdir. Bu nedenle tiyoredoksin redüktaz enziminin inhibisyonunun kanseri önlediği veya durdurduğu düşünülmektedir. Bu çalışmada, yayla balının, çam balının, kestane balının, deli veya yabani balının, polenin, propolisin ve arı sütünün ekstraktlarının tioredoksin redüktaz enzim aktivitesi üzerindeki etkileri araştırılmıştır. Enzim aktiviteleri IC50 değerlerini hesaplamak için sabit substrat ve farklı inhibitör konsantrasyonlarında ölçülmüştür. İnhibisyon sabitinin ölçümü için beşten fazla farklı inhibitör konsantrasyonları kullanılmıştır. İnhibisyon çalışmasında kullanılan ekstraktları karşılaştırmak için toplam antioksidan aktivitesi araştırılmıştır. En güçlü inhibisyon etkisi polen metanol ekstraktında (IC50 = 2.44 μg/mL) görülmüştür.

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Birincil Dil en
Konular Mühendislik, Kimya, Gıda Bilimi ve Teknolojisi, Temel Bilimler
Yayımlanma Tarihi December 2018
Dergi Bölümü Araştırma Makalesi
Yazarlar

Yazar: Ebru AKKEMİK
Kurum: SİİRT ÜNİVERSİTESİ
Ülke: Turkey


Yazar: Gamze AKBULUT
Ülke: Turkey


Bibtex @araştırma makalesi { saufenbilder341372, journal = {Sakarya University Journal of Science}, issn = {1301-4048}, eissn = {2147-835X}, address = {Sakarya Üniversitesi}, year = {2018}, volume = {22}, pages = {1585 - 1590}, doi = {10.16984/saufenbilder.341372}, title = {An Investigation of Inhibition Effects of Honey, Pollen, Propolis and Royal Jelly Extracts on Thioredoxin Reductase Enzyme Activity}, key = {cite}, author = {AKBULUT, Gamze and AKKEMİK, Ebru} }
APA AKKEMİK, E , AKBULUT, G . (2018). An Investigation of Inhibition Effects of Honey, Pollen, Propolis and Royal Jelly Extracts on Thioredoxin Reductase Enzyme Activity. Sakarya University Journal of Science, 22 (6), 1585-1590. DOI: 10.16984/saufenbilder.341372
MLA AKKEMİK, E , AKBULUT, G . "An Investigation of Inhibition Effects of Honey, Pollen, Propolis and Royal Jelly Extracts on Thioredoxin Reductase Enzyme Activity". Sakarya University Journal of Science 22 (2018): 1585-1590 <http://www.saujs.sakarya.edu.tr/issue/31266/341372>
Chicago AKKEMİK, E , AKBULUT, G . "An Investigation of Inhibition Effects of Honey, Pollen, Propolis and Royal Jelly Extracts on Thioredoxin Reductase Enzyme Activity". Sakarya University Journal of Science 22 (2018): 1585-1590
RIS TY - JOUR T1 - An Investigation of Inhibition Effects of Honey, Pollen, Propolis and Royal Jelly Extracts on Thioredoxin Reductase Enzyme Activity AU - Ebru AKKEMİK , Gamze AKBULUT Y1 - 2018 PY - 2018 N1 - doi: 10.16984/saufenbilder.341372 DO - 10.16984/saufenbilder.341372 T2 - Sakarya University Journal of Science JF - Journal JO - JOR SP - 1585 EP - 1590 VL - 22 IS - 6 SN - 1301-4048-2147-835X M3 - doi: 10.16984/saufenbilder.341372 UR - http://dx.doi.org/10.16984/saufenbilder.341372 Y2 - 2018 ER -
EndNote %0 Sakarya University Journal of Science An Investigation of Inhibition Effects of Honey, Pollen, Propolis and Royal Jelly Extracts on Thioredoxin Reductase Enzyme Activity %A Ebru AKKEMİK , Gamze AKBULUT %T An Investigation of Inhibition Effects of Honey, Pollen, Propolis and Royal Jelly Extracts on Thioredoxin Reductase Enzyme Activity %D 2018 %J Sakarya University Journal of Science %P 1301-4048-2147-835X %V 22 %N 6 %R doi: 10.16984/saufenbilder.341372 %U 10.16984/saufenbilder.341372
ISNAD AKKEMİK, Ebru , AKBULUT, Gamze . "Tiyoredoksin Redüktaz Enzim Aktivitesi Üzerine Bal, Polen, Propolis ve Arı Sütü Extraktlarının İnhibisyon Etkilerinin Araştırılması". Sakarya University Journal of Science 22 / 6 (Aralık 2018): 1585-1590. http://dx.doi.org/10.16984/saufenbilder.341372