Year 2020, Volume 24 , Issue 1, Pages 189 - 196 2020-02-01

Determination of Cytotoxic effect and Expression analyses of Apoptotic and Autophagic related genes in Thymoquinone-treated Colon Cancer Cells

Esra TOKAY [1]


Thymoquinone (Tq; 2-isopropyl-5-methyl-1,4 benzoquinone) is the main ingredient present in the volatile oil of Nigella sativa (black seed). Recently, it has been reported that, Tq was inhibited cell proliferation of many cancer cell lines including ovarian, lung, breast, osteosarcoma, pancreatic, fibrosarcoma, lung cancer, squamous cell carcinoma and myeloblastic leukemias. In this study, the anticancer effect of Tq was investigated in human Colon Cancer Cells (HT-29) with MTT assay. Positive control was achieved with 5-fluorouracil (5-FU). Also, the invasion capability of HT-29 cellls was determined in presence of Tq and 5-FU with scratch analyses. In addition, the autophagic and apoptotic effect of Tq were investigated by qRT-PCR method to find out the pathway of anti-proliferative effect in Tq-treated HT-29 cells. As a result, Tq was inhibited the cell proliferation of HT-29 cells in time dependent manner, with 118  µM for 24 h and 84 µM for 48 h, respectively. A concentration of 150 µM of Tq was able to significantly reduced the invasiveness of HT-29 cells about  ̴8 fold compared with not treated cells at 6h. ATG-12, ATG-7 and LC3-II were significantly downregulated in presence of Tq.  Conversaly, the expression of all of the autophagy genes were downregulated in presence of 5-FU. Moreover, pro-apoptotic gene Bax was significiantly upregulated nearly 15 fold whereas the expressions of Bcl-2 and Bcl-XL (pro-survival genes) were decreased in presence of Tq. On the other hand, in 5-FU treated HT-29 cells, the expression of  Bax, Bcl-2 and Bcl-XL genes and autophagy related genes were significantly downregulated. 

Tq, autophagy, apoptosis, HT-29
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Primary Language en
Subjects Biology
Published Date February 2020
Journal Section Research Articles
Authors

Orcid: 0000-0001-9993-2753
Author: Esra TOKAY (Primary Author)
Institution: BALIKESIR UNIVERSITY
Country: Turkey


Supporting Institution Balıkesir Üniversitesi
Dates

Application Date : July 1, 2019
Acceptance Date : December 2, 2019
Publication Date : February 1, 2020

Bibtex @research article { saufenbilder585012, journal = {Sakarya University Journal of Science}, issn = {1301-4048}, eissn = {2147-835X}, address = {}, publisher = {Sakarya University}, year = {2020}, volume = {24}, pages = {189 - 196}, doi = {10.16984/saufenbilder.585012}, title = {Determination of Cytotoxic effect and Expression analyses of Apoptotic and Autophagic related genes in Thymoquinone-treated Colon Cancer Cells}, key = {cite}, author = {TOKAY, Esra} }
APA TOKAY, E . (2020). Determination of Cytotoxic effect and Expression analyses of Apoptotic and Autophagic related genes in Thymoquinone-treated Colon Cancer Cells. Sakarya University Journal of Science , 24 (1) , 189-196 . DOI: 10.16984/saufenbilder.585012
MLA TOKAY, E . "Determination of Cytotoxic effect and Expression analyses of Apoptotic and Autophagic related genes in Thymoquinone-treated Colon Cancer Cells". Sakarya University Journal of Science 24 (2020 ): 189-196 <http://www.saujs.sakarya.edu.tr/en/issue/49430/585012>
Chicago TOKAY, E . "Determination of Cytotoxic effect and Expression analyses of Apoptotic and Autophagic related genes in Thymoquinone-treated Colon Cancer Cells". Sakarya University Journal of Science 24 (2020 ): 189-196
RIS TY - JOUR T1 - Determination of Cytotoxic effect and Expression analyses of Apoptotic and Autophagic related genes in Thymoquinone-treated Colon Cancer Cells AU - Esra TOKAY Y1 - 2020 PY - 2020 N1 - doi: 10.16984/saufenbilder.585012 DO - 10.16984/saufenbilder.585012 T2 - Sakarya University Journal of Science JF - Journal JO - JOR SP - 189 EP - 196 VL - 24 IS - 1 SN - 1301-4048-2147-835X M3 - doi: 10.16984/saufenbilder.585012 UR - https://doi.org/10.16984/saufenbilder.585012 Y2 - 2019 ER -
EndNote %0 Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi Determination of Cytotoxic effect and Expression analyses of Apoptotic and Autophagic related genes in Thymoquinone-treated Colon Cancer Cells %A Esra TOKAY %T Determination of Cytotoxic effect and Expression analyses of Apoptotic and Autophagic related genes in Thymoquinone-treated Colon Cancer Cells %D 2020 %J Sakarya University Journal of Science %P 1301-4048-2147-835X %V 24 %N 1 %R doi: 10.16984/saufenbilder.585012 %U 10.16984/saufenbilder.585012
ISNAD TOKAY, Esra . "Determination of Cytotoxic effect and Expression analyses of Apoptotic and Autophagic related genes in Thymoquinone-treated Colon Cancer Cells". Sakarya University Journal of Science 24 / 1 (February 2020): 189-196 . https://doi.org/10.16984/saufenbilder.585012
AMA TOKAY E . Determination of Cytotoxic effect and Expression analyses of Apoptotic and Autophagic related genes in Thymoquinone-treated Colon Cancer Cells. SAUJS. 2020; 24(1): 189-196.
Vancouver TOKAY E . Determination of Cytotoxic effect and Expression analyses of Apoptotic and Autophagic related genes in Thymoquinone-treated Colon Cancer Cells. Sakarya University Journal of Science. 2020; 24(1): 196-189.