Year 2020, Volume 24 , Issue 1, Pages 265 - 271 2020-02-01

Effect of Mitosis on the Resistance to Oxidative and Osmotic Stresses in Yeast

Pinar Buket ATALAY [1] , Nur KALUC [2] , Elif Ergin CAVUSOGLU [3]


Cancer cells are defined by abnormal and unrestricted mitotic divisions, therefore targeting mitosis is a useful strategy for cancer treatment. Two groups of drugs that are most successfully used in the treatment of several types of cancer, taxanes and vinca alkaloids, exhibit their anti-tumor effects by causing a mitotic arrest. However, not much is known about whether being arrested in mitosis affects the sensitivity of cells to tumor-related stresses, such as oxidative and osmotic stresses. In this study, we investigated whether mitosis affects the sensitivity of Saccharomyces cerevisiae cells to H2O2-induced oxidative stress and sorbitol-induced osmotic stress. Mitotic and G1-arrests were induced by nocodazole and alpha factor, respectively. The effects of nocodazole or alpha factor treatments on the sensitivity of wild type (WT) and MAD3 deletion (mad3Δ) strains to oxidative stress and osmotic stress were evaluated by the spotting and coloni forming unit (cfu) assays as well as detection of reactive oxygen species (ROS) production. Data were analyzed using Student’s t-test and expressed as standard deviation (std), p<0.05 was considered significant. Our data indicate that mitosis significantly increases resistance to oxidative stress, however it does not have any significant effect on the osmotic stress resistance in yeast.

Oxidative stress, Osmotic stress, Mitosis, Saccharomyces cerevisiae
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Primary Language en
Subjects Biology
Published Date February 2020
Journal Section Research Articles
Authors

Orcid: 0000-0001-7627-0291
Author: Pinar Buket ATALAY (Primary Author)
Institution: FACULTY OF MEDICINE
Country: Turkey


Orcid: 0000-0002-7323-3150
Author: Nur KALUC
Institution: FACULTY OF MEDICINE
Country: Turkey


Orcid: 0000-0002-0573-6047
Author: Elif Ergin CAVUSOGLU
Institution: Marmara University Institute of Health Sciences
Country: Turkey


Dates

Application Date : May 2, 2019
Acceptance Date : October 17, 2019
Publication Date : February 1, 2020

Bibtex @research article { saufenbilder559820, journal = {Sakarya University Journal of Science}, issn = {1301-4048}, eissn = {2147-835X}, address = {}, publisher = {Sakarya University}, year = {2020}, volume = {24}, pages = {265 - 271}, doi = {10.16984/saufenbilder.559820}, title = {Effect of Mitosis on the Resistance to Oxidative and Osmotic Stresses in Yeast}, key = {cite}, author = {ATALAY, Pinar Buket and KALUC, Nur and CAVUSOGLU, Elif Ergin} }
APA ATALAY, P , KALUC, N , CAVUSOGLU, E . (2020). Effect of Mitosis on the Resistance to Oxidative and Osmotic Stresses in Yeast. Sakarya University Journal of Science , 24 (1) , 265-271 . DOI: 10.16984/saufenbilder.559820
MLA ATALAY, P , KALUC, N , CAVUSOGLU, E . "Effect of Mitosis on the Resistance to Oxidative and Osmotic Stresses in Yeast". Sakarya University Journal of Science 24 (2020 ): 265-271 <http://www.saujs.sakarya.edu.tr/en/issue/49430/559820>
Chicago ATALAY, P , KALUC, N , CAVUSOGLU, E . "Effect of Mitosis on the Resistance to Oxidative and Osmotic Stresses in Yeast". Sakarya University Journal of Science 24 (2020 ): 265-271
RIS TY - JOUR T1 - Effect of Mitosis on the Resistance to Oxidative and Osmotic Stresses in Yeast AU - Pinar Buket ATALAY , Nur KALUC , Elif Ergin CAVUSOGLU Y1 - 2020 PY - 2020 N1 - doi: 10.16984/saufenbilder.559820 DO - 10.16984/saufenbilder.559820 T2 - Sakarya University Journal of Science JF - Journal JO - JOR SP - 265 EP - 271 VL - 24 IS - 1 SN - 1301-4048-2147-835X M3 - doi: 10.16984/saufenbilder.559820 UR - https://doi.org/10.16984/saufenbilder.559820 Y2 - 2019 ER -
EndNote %0 Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi Effect of Mitosis on the Resistance to Oxidative and Osmotic Stresses in Yeast %A Pinar Buket ATALAY , Nur KALUC , Elif Ergin CAVUSOGLU %T Effect of Mitosis on the Resistance to Oxidative and Osmotic Stresses in Yeast %D 2020 %J Sakarya University Journal of Science %P 1301-4048-2147-835X %V 24 %N 1 %R doi: 10.16984/saufenbilder.559820 %U 10.16984/saufenbilder.559820
ISNAD ATALAY, Pinar Buket , KALUC, Nur , CAVUSOGLU, Elif Ergin . "Effect of Mitosis on the Resistance to Oxidative and Osmotic Stresses in Yeast". Sakarya University Journal of Science 24 / 1 (February 2020): 265-271 . https://doi.org/10.16984/saufenbilder.559820
AMA ATALAY P , KALUC N , CAVUSOGLU E . Effect of Mitosis on the Resistance to Oxidative and Osmotic Stresses in Yeast. SAUJS. 2020; 24(1): 265-271.
Vancouver ATALAY P , KALUC N , CAVUSOGLU E . Effect of Mitosis on the Resistance to Oxidative and Osmotic Stresses in Yeast. Sakarya University Journal of Science. 2020; 24(1): 271-265.