Year 2016, Volume 20 , Issue 2, Pages 245 - 250 2016-08-01

Characterization of polyphenol oxidase from red deadnettle (lamium purpureum) and its inhibition
Eflatun çiçekli ballıbaba (lamium purpureum) polifenol oksidaz enziminin karakterizasyonu ve inhibisyonu

Elif Cerrahoğlu [1] , Gülnur Arabacı [2]


In this work, it is investigated the kinetic properties of polyphenol oxidase (PPO) obtained from Lamium purpureum (red deadnettle).  4-methylcatechol was used as substrate in the characterization studies. Michaelis-Menten constant (KM) and maximum reaction velocity (Vmax) were calculated. Enzyme’s optimum pH value was found 7,5; optimum temperature value was found 10 . KM and Vmax values were calculated 2,9977 mM and 0,0087 EU/min respectively. PPO activity substantially was inhibited from sodium azide, thiourea, L-cysteine, ascorbic acid, benzoic acid, citric acid, 2-mercaptoethanol and I50 values were calculated each inhibitor.

Bu çalışmada, Lamium purpureum (eflatun çiçekli ballıbaba) bitkisinden elde edilen polifenol oksidaz (PPO) enziminin kinetik özellikleri incelenmiştir. Karakterizasyon çalışmalarında substrat olarak 4-metil katekol kullanılmıştır. 4-metil katekol için Michaelis-Menten sabiti (KM) ve maksimum reaksiyon hızı (Vmax) hesaplanmıştır. Enziminin optimum pH değeri 7,5, optimum sıcaklık değerleri ise 10 °C bulunmuştur. Vmax ve KM değerleri ise sırasıyla 2,9977 mM ve 0,0087 EU/dak olarak hesaplanmıştır. PPO enzimi için sodyum azid, tiyoüre, L-Sistein, askorbik asit, sitrik asit, benzoik asit, 2-merkaptoetanol ile inhibisyon çalışması yapılmış, her bir inhibitör için I50 değeri hesaplanmıştır.

Subjects Engineering
Published Date Ağustos 2016
Journal Section Research Articles
Authors

Author: Elif Cerrahoğlu

Author: Gülnur Arabacı

Dates

Application Date : April 25, 2014
Acceptance Date : February 3, 2016
Publication Date : August 1, 2016

Bibtex @research article { saufenbilder261128, journal = {Sakarya University Journal of Science}, issn = {1301-4048}, eissn = {2147-835X}, address = {}, publisher = {Sakarya University}, year = {2016}, volume = {20}, pages = {245 - 250}, doi = {10.16984/saufenbilder.80440}, title = {Characterization of polyphenol oxidase from red deadnettle (lamium purpureum) and its inhibition}, key = {cite}, author = {Cerrahoğlu, Elif and Arabacı, Gülnur} }
APA Cerrahoğlu, E , Arabacı, G . (2016). Characterization of polyphenol oxidase from red deadnettle (lamium purpureum) and its inhibition. Sakarya University Journal of Science , 20 (2) , 245-250 . DOI: 10.16984/saufenbilder.80440
MLA Cerrahoğlu, E , Arabacı, G . "Characterization of polyphenol oxidase from red deadnettle (lamium purpureum) and its inhibition". Sakarya University Journal of Science 20 (2016 ): 245-250 <http://www.saujs.sakarya.edu.tr/en/issue/24694/261128>
Chicago Cerrahoğlu, E , Arabacı, G . "Characterization of polyphenol oxidase from red deadnettle (lamium purpureum) and its inhibition". Sakarya University Journal of Science 20 (2016 ): 245-250
RIS TY - JOUR T1 - Characterization of polyphenol oxidase from red deadnettle (lamium purpureum) and its inhibition AU - Elif Cerrahoğlu , Gülnur Arabacı Y1 - 2016 PY - 2016 N1 - doi: 10.16984/saufenbilder.80440 DO - 10.16984/saufenbilder.80440 T2 - Sakarya University Journal of Science JF - Journal JO - JOR SP - 245 EP - 250 VL - 20 IS - 2 SN - 1301-4048-2147-835X M3 - doi: 10.16984/saufenbilder.80440 UR - https://doi.org/10.16984/saufenbilder.80440 Y2 - 2016 ER -
EndNote %0 Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi Characterization of polyphenol oxidase from red deadnettle (lamium purpureum) and its inhibition %A Elif Cerrahoğlu , Gülnur Arabacı %T Characterization of polyphenol oxidase from red deadnettle (lamium purpureum) and its inhibition %D 2016 %J Sakarya University Journal of Science %P 1301-4048-2147-835X %V 20 %N 2 %R doi: 10.16984/saufenbilder.80440 %U 10.16984/saufenbilder.80440
ISNAD Cerrahoğlu, Elif , Arabacı, Gülnur . "Characterization of polyphenol oxidase from red deadnettle (lamium purpureum) and its inhibition". Sakarya University Journal of Science 20 / 2 (August 2016): 245-250 . https://doi.org/10.16984/saufenbilder.80440
AMA Cerrahoğlu E , Arabacı G . Characterization of polyphenol oxidase from red deadnettle (lamium purpureum) and its inhibition. SAUJS. 2016; 20(2): 245-250.
Vancouver Cerrahoğlu E , Arabacı G . Characterization of polyphenol oxidase from red deadnettle (lamium purpureum) and its inhibition. Sakarya University Journal of Science. 2016; 20(2): 250-245.